Western blot assay of COX-1 and COX-2 expression by macrophage (Raw 264.7. 20) cells after LPS stimulation. For each lane of SDS polyacrylamide gels, 20 μg of protein was loaded. COX-1 and COX-2. The proteins were detected on nitrocellulose membranes using specific antibodies and visualized by enhanced chemiluminescence. (A) COX-1: VC = vehicle control; S = separation lane (not loaded with protein); D1 = dexamethasone (100 μM); D2 = dexamethasone (10 μM); R1 = RCM-101 (100 μg/mL); R2 = RCM-101 (10 μg/mL); PC = positive control (COX-1 lysate). (B) COX-2: VC = vehicle control; B = cells not stimulated with LPS; R = RCM-101 (100 μg/mL); D = dexamethasone (100 μM); PC = positive control (COX-2 lysate).