| |
aDPPH radical scavenging IC50 (mg/ml)
|
bImmunomodulatory index in vitro
|
cImmunomodulatory index ex vivo
|
|---|
|
Control
| |
1.00 ± 0.03
|
1.00 ± 0.05
|
|
Yang herbs
| | | |
|
Cortex Eucommiae
|
> 5
|
0.46 ± 0.02*
|
1.04 ± 0.09
|
|
Fructus Psoraleae
|
1.0 ± 0.0
|
1.42 ± 0.02*
|
1.23 ± 0.01*
|
|
Herba Cistanches
|
1.8 ± 0.0
|
0.75 ± 0.13
|
0.95 ± 0.12
|
|
Herba Epimedii
|
1.1 ± 0.1
|
0.57 ± 0.03*
|
0.98 ± 0.01
|
|
Radix Dipsaci
|
0.8 ± 0.0
|
0.42 ± 0.08*
|
1.02 ± 0.02
|
|
Radix Morindae
|
> 5
|
2.16 ± 0.10*
|
1.31 ± 0.04*
|
|
Rhizoma Cibotii
|
0.6 ± 0.0
|
0.16 ± 0.03*
|
0.97 ± 0.04
|
|
Rhizoma Drynariae
|
> 5
|
0.59 ± 0.09*
|
0.98 ± 0.08
|
|
Yin herbs
| | | |
|
Fructus Ligustri
|
0.5 ± 0.0
|
1.73 ± 0.07*
|
1.80 ± 0.17*
|
|
Herba Dendrobii
|
1.4 ± 0.1
|
2.54 ± 0.09*
|
1.59 ± 0.09*
|
|
Herba Ecliptae
|
> 5
|
1.65 ± 0.02*
|
1.27 ± 0.12*
|
|
Radix Asparagi
|
> 5
|
0.70 ± 0.02*
|
1.24 ± 0.05*
|
|
Radix Ophiopogonis
|
> 5
|
1.65 ± 0.05*
|
1.44 ± 0.11*
|
|
Radix Oryzae
|
> 5
|
0.78 ± 0.13
|
0.97 ± 0.04
|
|
Rhizoma Polygonati
|
3.5 ± 0.3
|
1.43 ± 0.09*
|
1.21 ± 0.06*
|
|
Semen Prinsepiae
|
> 5
|
1.70 ± 0.03*
|
1.39 ± 0.11*
|
- aMethanol extracts of tonifying herbs were subjected to the DPPH assay. Values given are means ± S.D., (n = 3). (DPPH scavenging activity was regarded as negligible if the IC50 was > 5 mg/ml).
- bSplenocytes isolated from mice were cultured in 96-well microtiter plates in a final volume of 100 μl of culture medium, with the respective methanol extracts added at final concentrations ranging from 15.6–1000 μg/ml. Values given are means ± S.E.M., (n = 4).
- cAnimals were pretreated orally with the methanol extracts at a daily dose of 1 g/kg for 3 days. All animals were sacrificed 24 hours post-dosing. Splenocytes isolated from pretreated animals were cultured in microtiter plates in a final volume of 100 μl culture medium. Values given are means ± S.E.M., (n = 3–5).
- * Significantly different from the control group (P < 0.05)
