Table 2 Regression equation, correlation coefficients, linearity ranges and limit of detection and quantitation for the markers of Gegen Tang granule
Compounds | Regression equation | Linear range (μg/mL) | R2 | LOD (ng/mL) | LOQ (ng/mL) | Intra-day RSD% (n = 6) | Inter-day RSD% (n = 3) |
|---|---|---|---|---|---|---|---|
Puerarin | y = 40149x – 5483 | 1.70 – 69.70 | 0.9997 | 6.21 | 20.70 | 0.83 | 0.78 |
Paeoniflorin | y = 13018x + 3168 | 1.07 – 64.36 | 0.9996 | 19.64 | 65.46 | 0.99 | 0.45 |
Cinnamic acid | y = 68413x + 2472 | 0.15 – 5.81 | 0.9998 | 3.72 | 12.40 | 0.61 | 0.60 |
Daidzain | y = 44698x + 350 | 0.06 – 4.96 | 0.9997 | 5.58 | 18.60 | 0.33 | 1.83 |
Glycyrrhizin | y = 5695x – 3089 | 1.07 – 85.26 | 0.9998 | 45.47 | 151.56 | 0.10 | 1.70 |
Ephedrine | y = 21411x +1949 | 1.08 – 86.13 | 0.9998 | 3.82 | 12.73 | 0.52 | 1.55 |
Pseudoephedrine | y = 30269x – 41321 | 2.98 – 119.13 | 0.9996 | 2.71 | 9.03 | 0.37 | 0.41 |