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Table 2 Regression equation, correlation coefficients, linearity ranges and limit of detection and quantitation for the markers of Gegen Tang granule

From: Simultaneous determination of multiple marker constituents in concentrated Gegen Tang granule by high performance liquid chromatography

Compounds Regression equation Linear range (μg/mL) R2 LOD (ng/mL) LOQ (ng/mL) Intra-day RSD% (n = 6) Inter-day RSD% (n = 3)
Puerarin y = 40149x – 5483 1.70 – 69.70 0.9997 6.21 20.70 0.83 0.78
Paeoniflorin y = 13018x + 3168 1.07 – 64.36 0.9996 19.64 65.46 0.99 0.45
Cinnamic acid y = 68413x + 2472 0.15 – 5.81 0.9998 3.72 12.40 0.61 0.60
Daidzain y = 44698x + 350 0.06 – 4.96 0.9997 5.58 18.60 0.33 1.83
Glycyrrhizin y = 5695x – 3089 1.07 – 85.26 0.9998 45.47 151.56 0.10 1.70
Ephedrine y = 21411x +1949 1.08 – 86.13 0.9998 3.82 12.73 0.52 1.55
Pseudoephedrine y = 30269x – 41321 2.98 – 119.13 0.9996 2.71 9.03 0.37 0.41
  1. All regression results are statistically significant (P < 0.05)