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Figure 2 | Chinese Medicine

Figure 2

From: Effects of Yinchenhao Tang and related decoctions on DMN-induced cirrhosis/fibrosis in rats

Figure 2

(a) Real-time quantitative PCR. In the 2-week DMN group, α-SMA mRNA expression level increased (vs control rats, P < 0.01). In the DMN + water group, α-SMA mRNA expression level also increased (vs control rats, P < 0.01). In the DMN + YCHT group, α-SMA mRNA expression level decreased (vs DMN + water rats, P < 0.05). P < 0.05 ▲▲ P < 0.01 (vs DMN+ water) P < 0.05 P < 0.01 (vs 2-week DMN). (b) Western blot. Western blot analysis of total protein extracts was performed with antibodies recognizing α-SMA and Glyceraldehyde 3-phosphate dehydrogenase (GAPDH). YCHT reduced α-SMA protein expression significantly (vs DMN + water). (c) Immunohistochemical analysis ×200. (A) In the control group, α-SMA-positive HSCs were weakly stained and only detected in vascular structures. (B) and (C) With DMN-intoxication, number of α-SMA-positive HSCs increased gradually and peaked at week 4. (D) In the DMN + YCHT group, number of α-SMA-positive HSCs was significantly reduced.

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