Simultaneous determination of eight major bioactive compounds in Dachengqi Tang (DT) by high-performance liquid chromatography

Table 1 Calibration of eight markers in Dachengqi Tang

Marker	Calibration	r²	P	Test range (μg/ml)	LOD (μg/ml)	LOQ (μg/ml)
Naringin	y = 0.2009 x - 0.2487	0.9996	<0.001	2.50–200	0.31	0.93
Hesperidin	y = 0.1996 x - 1.3249	0.9982	<0.001	6.95–556	0.43	1.74
Aloe emodin	y = 0.1794 x - 0.267	0.9994	<0.001	1.83–146	0.23	0.92
Rhein	y = 0.1728 x - 0.039	0.9996	<0.001	1.75–140	0.21	0.84
Honokial	y = 0.1388 x - 0.0792	0.9992	<0.001	1.05–84	0.25	0.81
Magnolol	y = 0.1363 x + 0.0025	1.0000	<0.001	2.07–166	0.23	1.03
Emodin	y = 0.4250 x + 0.0452	0.9992	<0.001	2.20–176	0.28	0.83
Chrysophanol	y = 0.2125 x – 0.0184	0.9998	<0.001	2.05–164	0.25	0.76

y: peak area ratio (analyte/internal standard); x: concentration of analyte (μg/ml); LOD (limit of detection): S/N = 3; LOQ (limit of quantification): RSD < 20%

ISSN: 1749-8546