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Table 1 Calibration of eight markers in Dachengqi Tang

From: Simultaneous determination of eight major bioactive compounds in Dachengqi Tang (DT) by high-performance liquid chromatography

Marker Calibration r2 P Test range (μg/ml) LOD (μg/ml) LOQ (μg/ml)
Naringin y = 0.2009 x - 0.2487 0.9996 <0.001 2.50–200 0.31 0.93
Hesperidin y = 0.1996 x - 1.3249 0.9982 <0.001 6.95–556 0.43 1.74
Aloe emodin y = 0.1794 x - 0.267 0.9994 <0.001 1.83–146 0.23 0.92
Rhein y = 0.1728 x - 0.039 0.9996 <0.001 1.75–140 0.21 0.84
Honokial y = 0.1388 x - 0.0792 0.9992 <0.001 1.05–84 0.25 0.81
Magnolol y = 0.1363 x + 0.0025 1.0000 <0.001 2.07–166 0.23 1.03
Emodin y = 0.4250 x + 0.0452 0.9992 <0.001 2.20–176 0.28 0.83
Chrysophanol y = 0.2125 x – 0.0184 0.9998 <0.001 2.05–164 0.25 0.76
  1. y: peak area ratio (analyte/internal standard); x: concentration of analyte (μg/ml); LOD (limit of detection): S/N = 3; LOQ (limit of quantification): RSD < 20%