|
Marker
|
Calibration
|
r2
|
P
|
Test range (μg/ml)
|
LOD (μg/ml)
|
LOQ (μg/ml)
|
|---|
|
Naringin
|
y = 0.2009 x - 0.2487
|
0.9996
|
<0.001
|
2.50–200
|
0.31
|
0.93
|
|
Hesperidin
|
y = 0.1996 x - 1.3249
|
0.9982
|
<0.001
|
6.95–556
|
0.43
|
1.74
|
|
Aloe emodin
|
y = 0.1794 x - 0.267
|
0.9994
|
<0.001
|
1.83–146
|
0.23
|
0.92
|
|
Rhein
|
y = 0.1728 x - 0.039
|
0.9996
|
<0.001
|
1.75–140
|
0.21
|
0.84
|
|
Honokial
|
y = 0.1388 x - 0.0792
|
0.9992
|
<0.001
|
1.05–84
|
0.25
|
0.81
|
|
Magnolol
|
y = 0.1363 x + 0.0025
|
1.0000
|
<0.001
|
2.07–166
|
0.23
|
1.03
|
|
Emodin
|
y = 0.4250 x + 0.0452
|
0.9992
|
<0.001
|
2.20–176
|
0.28
|
0.83
|
|
Chrysophanol
|
y = 0.2125 x – 0.0184
|
0.9998
|
<0.001
|
2.05–164
|
0.25
|
0.76
|
- y: peak area ratio (analyte/internal standard); x: concentration of analyte (μg/ml); LOD (limit of detection): S/N = 3; LOQ (limit of quantification): RSD < 20%
