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Table 3 Intra-day variability for the simultaneous determination of eight markers in Dachengqi Tang (n = 6)

From: Simultaneous determination of eight major bioactive compounds in Dachengqi Tang (DT) by high-performance liquid chromatography

Marker Concentration (μg/ml) Measured concentration (μg/ml, mean (SD)) Accuracya (%) RSDb (%)
Naringin 25.0 24.87 (0.29) 99.5 1.2
62.5 62.80 (1.26) 100.5 2.0
156.3 155.53 (3.02) 99.5 1.9
Hesperidin 69.5 69. 79 (1.38) 100.4 2.0
173.8 172.14 (3.62) 99.0 2.1
434.4 429.38 (4.62) 98.8 1.1
Aloe emodin 18.3 18.72 (0.53) 102.3 2.8
45.8 46.79 (1.58) 102.2 3.4
114.4 112.77 (3.56) 98.6 3.2
Rhein 17.5 17.81 (0.21) 101.8 1.2
43.8 43.83 (0.69) 100.1 1.6
109.4 108.86 (1.55) 99.5 1.4
Honokial 10.5 10.25 (0.11) 97.6 1.0
26.3 26.18 (0.22) 99.5 0.8
65.6 66.24 (1.53) 101.0 2.3
Magnolol 21.0 21.06 (0.47) 100.3 2.2
52.5 53.10 (1. 58) 101.1 3.0
131.3 131.16 (2.06) 99.9 1.6
Emodin 11.0 11.39 (0.24) 103.5 2.1
27.5 26.54 (0.71) 96.5 2.7
68.8 68.50 (1.34) 99.6 1.9
Chrysophanol 10.3 10.52 (0.23) 102.1 2.2
25.8 25.57 (0.41) 99.1 1.6
64.4 65.55 (1.17) 101.8 1.8
  1. a Accuracy (%) = mean of measured concentration/added concentration × 100
  2. b RSD (%) (relative standard deviation) = (SD/mean) × 100