Table 3 Intra-day variability for the simultaneous determination of eight markers in Dachengqi Tang (n = 6)
Marker | Concentration (μg/ml) | Measured concentration (μg/ml, mean (SD)) | Accuracya (%) | RSDb (%) |
|---|---|---|---|---|
Naringin | 25.0 | 24.87 (0.29) | 99.5 | 1.2 |
62.5 | 62.80 (1.26) | 100.5 | 2.0 | |
156.3 | 155.53 (3.02) | 99.5 | 1.9 | |
Hesperidin | 69.5 | 69. 79 (1.38) | 100.4 | 2.0 |
173.8 | 172.14 (3.62) | 99.0 | 2.1 | |
434.4 | 429.38 (4.62) | 98.8 | 1.1 | |
Aloe emodin | 18.3 | 18.72 (0.53) | 102.3 | 2.8 |
45.8 | 46.79 (1.58) | 102.2 | 3.4 | |
114.4 | 112.77 (3.56) | 98.6 | 3.2 | |
Rhein | 17.5 | 17.81 (0.21) | 101.8 | 1.2 |
43.8 | 43.83 (0.69) | 100.1 | 1.6 | |
109.4 | 108.86 (1.55) | 99.5 | 1.4 | |
Honokial | 10.5 | 10.25 (0.11) | 97.6 | 1.0 |
26.3 | 26.18 (0.22) | 99.5 | 0.8 | |
65.6 | 66.24 (1.53) | 101.0 | 2.3 | |
Magnolol | 21.0 | 21.06 (0.47) | 100.3 | 2.2 |
52.5 | 53.10 (1. 58) | 101.1 | 3.0 | |
131.3 | 131.16 (2.06) | 99.9 | 1.6 | |
Emodin | 11.0 | 11.39 (0.24) | 103.5 | 2.1 |
27.5 | 26.54 (0.71) | 96.5 | 2.7 | |
68.8 | 68.50 (1.34) | 99.6 | 1.9 | |
Chrysophanol | 10.3 | 10.52 (0.23) | 102.1 | 2.2 |
25.8 | 25.57 (0.41) | 99.1 | 1.6 | |
64.4 | 65.55 (1.17) | 101.8 | 1.8 |