Transcriptional activation of ApoA-IV mRNA by lipid micelles. A: Caco-2/TC7 cells were treated with lipid micelles (1:1000 and 1:2500) for 24 hours. Total RNAs were extracted and reverse transcribed to cDNA for real-time PCR analysis. The mRNA levels of ApoA-IV were determined by the Ct-value method and normalized by mRNA level of a house keeping gene 18S rRNA. B: Caco-2/TC7 cells were treated with various concentrations of lipid micelles for 48 hours. Cultures were collected for luciferase assay to determine ApoA-IV transcription. C: Caco-2/TC7 cells were treated with lipid micelles (1:2500 and 1:2000) for 24, 48 and 72 hours to measure the time-dependent regulation of ApoA-IV. Data are expressed as mean ± SD of the multiple of Basal (i.e. buffer-treated control set as one) and number of independent experiments (n) = 5.