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Table 4 The IC50 values of NK-B transactivation and pro-inflammatory mediators of the compounds isolated or semi-synthesized from A. paniculat a EtOAc extract

From: Isolation and identification of bioactive compounds in Andrographis paniculata (Chuanxinlian)

Compounds NF-κB TNFα IL-6 MIP-2 NO
Semi-synthetic analogues    (μg/ml)   
   3,19-O-diacetylanhydroandrographolide 2.2 2.89 2.08 1.29 2.02
   19-O-acetylanhydroandrographolide 2.4 3.85 2.75 2.29 2.08
Diterpenoids      
   14-deoxy-14,15-dehydroandrographolide 2.0 2.18 2.14 2.07 2.05
   19-O-acetyl-14-deoxy-11,12-didehydroandrographolide 4.4 5.07 3.94 4.41 4.11
Synthetic analogues      
   hexahydro-14-dehydroxyandrographolide 4.2 5.2 3.78 4.52 4.24
   3,19-dioxolabda-8(17),11E, 13-trien-16,15-olide 4.1 5.78 3.97 4.67 4.52
Steroids      
   ergosterol peroxide 4.7 4.71 5.32 4.55 4.67
   β-sitosterol and stigmasterol 5.2 5.34 5.97 4.88 4.55
Flavonoids      
   5-hydroxy-7,8-dimethoxyflavone 6.1 4.33 5.34 3.63 5.11
   5-hydroxy-7,8-dimethoxyflavanone 6.7 4.37 5.14 4.01 4.57
Diterpenoids      
   andrographolide (major compound) 9.4 5.62 8.93 5.48 8.48
   14-deoxy-11,12-didehydroandrographolide 17.1 20.64 23.6 15.03 11.26
  1. NF-κB trans-activation activity was assay as following: RAW 264.7 macrophages transiently transfected with NF-κB reporter plasmids were pretreated various compounds and then stimulated with LPS 100 ng/mL/IFN-γ 1000 units/mL for further was estimated by the Dual-Glo Luciferase reporter assay. The collected cell supernatants were assayed for TNFα, IL-6, and MIP-2 productions using commercial ELISA kits. NO was determined by Griess assay [17].