Figure 3

Effect of TXL on cytochrome c. Total protein from HT-29 treated with 1%TXL for 12, 24 and 48 hours (A) or indicated concentration of TXL for 48 hours (B) were analyzed by Western blot with specific antibodies against Bax and Bcl-2. Protein from cytosolic fraction of HT-29 which has been treated with TXL (1%) for 12, 24 and 48 hours (C) or indicated concentration of TXL for 48 hours (D) were analyzed by Western blot with specific antibodies against cytochrome c. GAPDH antibody was used as control for equal loading. The relative expressions of proteins were quantified using Bio-Rad Quatity-One software. Results are expressed as mean and standard deviation (n = 3),*P < 0.05 **P < 0.01compared with control group.