Figure 3

MS and UV/VIS Detection of PHY906. Three detection modes were employed to detect the spectrum of phytochemicals in PHY906 extracts. The top panel illustrates detection in the UV/VIS range using a photo diode array detector (200-400 nm). The middle panel illustrates detection by MS(+) with a TIC of 1.5e4. The lower panel illustrates detection by MS(-) with a TIC of 2.5e3. UV/VIS detection was poor for many of the saponins and triterpenoids associated with (G) and was unable to detect or resolve the marker for (Z) in the solvent front. Only 22 of the 39 peaks in the final chemical fingerprint were detected and no new peaks were observed. MS(+) detection was approximately eight fold more sensitive than MS(-) by TIC resulting in increased S/N. 32 of the 39 chemical fingerprint peaks were observed in the MS(-) mode compared with the MS(+) mode. No new peaks were observed in the MS(-) mode although the intensity profile was enhanced for a few species including paeoniflorin sulfonate at 25.6 minutes.