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Figure 5 | Chinese Medicine

Figure 5

From: A comprehensive platform for quality control of botanical drugs (PhytomicsQC): a case study of Huangqin Tang (HQT) and PHY906

Figure 5

LC/MS chromatogram of PHY906-6. LC/MS(+) spectrum of PHY906 extract and herbal source identification. Thirty-six peaks were resolved and 64 compounds were identified or tentatively identified (23). Thirty-one peaks were found to contain a single molecular species while 5 peaks contain multiple molecular species. 39 compound peaks defined the chemical fingerprint and were used for batch-to-batch comparisons. Of the 39 peaks of the chemical fingerprint of PHY906 (S) accounted for 25 of 39 peaks, (P) accounted for 3 of 39 peaks, (G) accounted for 10 of 39 peaks and (Z) accounted for only 1 of 39 peaks. All the identified peaks had a unique retention time and/or mass signature and were associated with a single herbal ingredient. Water extracted (Z) was nearly devoid of resolved phytochemical peaks that could be detected. The single identified peak for (Z) was very hydrophilic, had no UV chromophore, eluted in the solvent front of the C18 reverse phase column and ionized only in (+) positive MS mode. The total ion count for the spectrum was 2.9e4. The complete chemical fingerprint of 39 peaks accounted for more than 82% of the total ions above a threshold of 1.5% of the largest peak.

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