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Table 2 PHY906 gene expression bioresponse in HepG2 cell-line

From: A comprehensive platform for quality control of botanical drugs (PhytomicsQC): a case study of Huangqin Tang (HQT) and PHY906

Protein name

Gene name

Cellular function

Fold change

Aldo-keto reductase family 1 member B10

AKR1B10

Metabolism

6.8

Carnitine palmitoyltransferase 1A

CYP1A1

Metabolism

405

Epithelial membrane protein 2

EMP2

Cell growth regulation

3.2

Glucose-6-phosphatase catalytic subunit

G6PC

Metabolism

12.3

Glutamate-cystein ligase catalytic subunit

GCLC

Metabolism

3.4

Growth differentiation factor 15

GDF15

Cell growth/differentiation

2.2

Hepcidin antimicrobial peptide

HAMP

Homeostasis, metabolism

4.9

Insulin-like growth factor binding protein 3

IGFBP-3-2

Hormone, Immune response,

3.3

Palladin

Palladin

Cell growth regulation

2.6

Serine/threonine protein kinase PIM1

PIM1

Signalling transduction and cell proliferation, oncogene

3

Sterile alpha motifs- and SH3 domain-containing protein 1

SASH1

Cell growth regulation

2.8

SERTA domain

SERTAD

transcriptional regulator

2.2

Solute carrier family 7 member 11

SLC7A11

Membrane transport protein

3.2

Son of sevenless homolog 1

SOS 1

Signalling transduction and cell death regulation

9.4

Tubulin, alpha 3

TUBA3

Signalling transduction and cell death regulation

-2.4

  1. PHY906-6, at the IC50 dose (0.85 g/ml dry weight), or control buffer was applied to a standardized cell culture of HepG2 cells for 24 hr. No cell death was observed by methylene blue staining. Cells were harvested and RNA was isolated from both PHY906-6 treated and control treated cells. The RNA was quantitated using qRT-PCR and standardized gene probes from Applied Biosystems Assays-On-Demand for the 15 genes in the gene signature. Fourteen of the fifteen genes were up-regulated. The genes coded for proteins with a variety of cellular functions. No information regarding cellular mechanisms of action of PHY906 could be inferred from these data, as the data indicated the cellular bioactivity of the entire extract rather than the bioavailable fraction. The qRT-PCR data, however, were reproducible in an independent experiment within approximately 30%.