Figure 4

Microscopic images of immunostaining. Representative microscopic photos of cleaved caspase-3 positive staining cells in hippocampus (a, b) and cerebellum (c, d, e). Caspase-3 immunostaining of hippocampus samples from (a) ischemia control group 2A and (b) ischemia with PZH treatment group 1A. (a) Caspase-3 positive cells (arrows) were seen at the molecular (M), pyramidal (Py) and polymorphic (P) layers of hippocampus from a rat of ischemia control group, ×200. (b) Few caspase-3 positive cells (arrow) were found mainly in the pyramidal layer of hippocampus from a rat of ischemia with PZH treatment group, ×200. Caspase-3 immunostaining of cerebellum samples from (c, d) ischemia control group 2A and ischemia with (e) PZH treatment group 1A. (c) Caspase-3 positive cells were seen at the Purkinje layer (arrows) of cerebellum from a rat of ischemia control group, ×200. (d) A larger magnification of (c) to show the caspase-3 positive cells (arrows), ×400. (e) Only isolated caspase-3 positive cells (arrow) were found along the Purkinje layer of cerebellum from a rat of ischemia with PZH treatment group, ×200.