Inhibitory effect of FLL aqueous extract on rat MSCs under adipogenic induction on day 21. (A) The number of adipocytes was counted after Oil-red O staining. Troglitazone (Tro, 20 μM) acted as positive control. Data are the mean ± SD (n = 3) from three independent experiments. Significant difference: * P < 0.05; ** P < 0.01; *** P < 0.001 for difference from respective baseline cultures without treatment. (B) Effect of FLL aqueous extract on the cell viability of P2 adipogenic differentiated rat MSCs. MSCs were treated with different concentrations of FLL extract for 21 days. The cell viability was estimated by MTT assay and the results are expressed as mean ± SD of three independent experiments in triplicate. (C) Adipocyte deposition at different treatment concentrations (0, 50 and 100 μg/mL) was visualized by Oil Red O staining on day 21.