Figure 4

Primer design for CAPS markers. A 0.55-kb fragment was amplified from the eight Boehmeria lines with primers CP-S62-f (5'-CTGAGGCGGGAGCTAGGATTTCAACTAA-3') and CP-S62-r (5'-GGGGGAAGTAGTGCAGCACATGAATATA-3'). For the CAPS analysis, the restriction enzymes that generate polymorphic patterns were found by the dCAPS Finder 2.0 software (http://helix.wustl.edu/dcaps/dcaps.html). Five restriction enzymes (i.e. Afl III, Bsr FI, Msp I, Drd I and Nde) were used.