Skip to main content

Table 5 Identification of markers in the HPLC fingerprint of the formula by LC-MS analysis

From: Quality assurance for Chinese herbal formulae: standardization of IBS-20, a 20-herb preparation

   

APCI MS Data (Positive Ion)

  

Peak

Identification

Time (min)

M/QMIPa

Other Peaks

MS2 of M/QMIP

Plant Sourceb

Content in formulation

(mg/kg)

1

Esculin

6.4

341 (M+H)+

179

179

FR

 

2

Chlorogenic acid

10.3

355 (M+H)+

163

163

AS

 

3

Aesculetin

12.0

179 (M+H)+

 

134, 123, 109

FR

310

4

Paeoniflorin

18.8

498 (M+H2O)+

301, 179

301, 179

PL

 

5

prim-O-Glucosylcimifugin

23.8

469 (M+H)+

 

307

SD

 

6

Magnoflorine

32.2

342 (M)+

 

297, 265

CC, PA

 

7

Liquiritin

35.9

419 (M+H)+

307, 257

 

GU

 

8

5-O-Methylvisamminoside

49.9

453 (M+H)+

291

290

SD

 

9

Hesperidin

52.0

610 (M)+

465, 449, 303

463

CR

1460

10

Columbamine

60.2

338 (M)+

 

323, 294

CC

 

11

Jatrorrhizine

61.3

338 (M)+

 

323, 294

CC

 

12

Epiberberine

61.3

336 (M)+

  

CC

 

13

Coptisine

61.4

320 (M)+

 

304, 292

CC

 

14

Palmatine

66.5

352 (M)+

 

337, 308

CC, PA

420

15

Berberine

67.3

336 (M)+

 

321, 292

CC, PA

1620

16

Glycyrrhizic acid

76.2

823 (M+H)+

647, 471, 453, 406

 

GU

780

17

Schisandrin

81.8

433 (M+H)+

415

 

SC

140

18

Honokiol

93.3

266 (M)+

263

 

MO

63

19

Magnolol

98.6

266 (M)+

261

 

MO

93

20

Schisandrin A

107.7

417 (M+H)+

 

402, 347, 316

SC

 
  1. Note: Peak number refers to the chromatographic fingerprint of the clinical preparation (Figure 4). Peaks 6, 10, 12, and13 were identified by LC-MS/MS2 analysis based on the literature values [17, 18] and other peaks were identified by comparison of authentic chemicals. Jatrorrhizine and epiberberine (peaks 11, 12) were co-eluted with the same retention times.
  2. a Molecular or quasi-molecular ion peak; b FR: Fraxinus rhynchophylla; AS: Artemisia scoparia; PL: Paeonia lactiflora; SD: Saposhinikovia divaricata; GU: Glycyrrhiza uralensis; CR: Citrus reticulata; CC: Coptis chinensis; PA: Phellodendron amurense; SC: Schisandra chinensis; MO: Magnolia officinalis.
Back to article page

Chinese Medicine

ISSN: 1749-8546

Contact us