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Figure 2 | Chinese Medicine

Figure 2

From: Effects of a multi-herbal extract on type 2 diabetes

Figure 2

Extract activates AMPK in C2C12 cells. (A) Extract increased AMPK phosphorylation. C2C12 cells were treated with aminoimidazole-4-carboxamide-1-β-d-ribofuranoside (1 mmol/l), extract (5 μg/ml), or macelignan (10 μM) for 24 hours. Phosphorylated AMPK was examined by Western blot analysis, (B) extract increased the mRNA expression of ACS, CPT-1. The expression was estimated using quantitative real-time RT-PCR. Data represent are shown as mean ± SD of three independent experiments (*P < 0.05, **P < 0.01, ***P < 0.001)

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