Figure 3

Extract inhibits NFkB signaling in HepG2 cells. (A) extract prevented the increase of TNF-α-stimulated luciferase activity in TNF-α treated HepG2. HepG2 cells were transfected with NFkB-Luc reporter and then treated with extract (5 μg/ml), rosiglitazone (10 μM), or macelignan (10 μM) for 24 hours in the presence of TNF-α (10 ng/ml) (B) extract increased the IkB level. HepG2 cells were preincubated with extract (5 μg/ml), rosiglitazone (10 μM), or macelignan (10 μM) for 24 hours and then treated with TNF-α (10 ng/ml) for one hour. IκBα was measured by Western blot analysis. Data represent are shown as mean ± SD of three independent experiments (*P < 0.05, **P < 0.01, ***P < 0.001)