(A) Effect of harmine on gene expression. B16F-10 cells were cultured in the presence and absence of harmine (2 μg/mL) and cDNA was synthesized and amplified with appropriate primers using PCR. The mRNA expression levels were normalized with GAPDH (house-keeping gene). (B) Effect of harmine on transcription factors. B16F-10 melanoma cells were treated with harmine (2 μg/mL) for two hours and then incubated with TNF-α (10 pg/mL) for 30 minutes. Percentage inhibition in various transcription factors were measured by ELISA method.