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Table 2 Effect of harmine on the release of TNF-α, IL-1β, IL-6 and GM CSF by B16F-10 melanoma cells

From: Harmine activates intrinsic and extrinsic pathways of apoptosis in B16F-10 melanoma

Cytokine (pg/mL)   Harmine
  Control 1 μg/mL 2 μg/mL
TNF-α 29.17 ± 5.94 26.18 ± 1.71 20.19 ± 1.12 (P = 0.012)
IL-1β 98.15 ± 2.08 74.48 ± 2.11 (P < 0.001) 61.34 ± 2.34 (P < 0.001)
IL-6 61.43 ± 4.44 40.42 ± 5.19 (P < 0.001) 28.46 ± 2.80 (P < 0.001)
GM-CSF 34.55 ± 1.07 29.55 ± 1.21 (P = 0.01) 22.81 ± 1.19 (P < 0.001)
  1. B16F-10 cells (5 × 103 cells) were cultured in the presence of harmine for 48 hours, and level of pro-inflammatory cytokines in the culture supernatant was estimated. Values are expressed as mean ± SD. Statistical analysis was performed with ANOVA, followed by Dunnet's test using GraphPad Instat software.