Effects of RPR-EA-S1 on the production of different cytokines or chemokines in BCG-stimulated PBMac. PBMac (5 × 105) were pretreated with 0.01% DMSO or 20 μg/mL of RPR-EA-S1 overnight and then stimulated with BCG (MOI = 1). Supernatants were collected after 24 hours. The levels of IL-6, IL-8, IL-10 and TNF-α in the supernatants were measured by ELISA. The individual cytokine level in the supernatant of DMSO + BCG sample was set as 100%, and the rest were compared to it to obtain a percentage value. Results are shown as mean ± SD from independent experiments on PBMac obtained from four different healthy donors. **P < 0.001 compared to the DMSO + BCG sample (one-way ANOVA, Tukey's test).