Figure 4
From: Array-based techniques for fingerprinting medicinal herbs
Method of manufacturing a subtracted diversity array and using it for fingerprinting herbal plants. The method shown in the figure is for subtraction of non-angiosperm DNA from angiosperm DNA which is a broad subtraction. This subtraction was capable of fingerprinting species from different angiosperm clades and orders with high (68%) polymorphism [20] but showed lower (10-22%) polymorphism when fingerprinting closely related species [36]. However, genomic DNA pool of species belonging to a particular family or order can be subtracted from genomic DNA pool of other family or order for a closer subtraction to obtain high polymorphism for closely related species [Mantri, unpublished data]. It is possible to use a single/dual-dye system where one sample can be a reference and other a test sample, or both samples can be test samples as shown in the figure.