Table 2 Nucleotide sequences of specific primers used for the amplification of the chloroplast DNA and PRK gene
From: Molecular identification of Lodoicea maldivica (coco de mer) seeds
Name | Oligonucleotide DNA sequence (5'-3') | Amplicon size | Specificity |
|---|---|---|---|
Plant 1 (forward) | CGA AAT CGG TAG ACG CTA CG | 550 bp | Chloroplast DNA |
Plant 2 (reverse) | GGG GAT AGA GGG ACT TGA AC | Â | Â |
prk717f (forward) | GTG ATA TGG AAG AAC GTG G | 750 bp | PRK gene |
prk969r (reverse) | ATT CCA GGG TAT GAG CAG C | Â | Â |