Figure 1
Nuclear morphological changes. Nuclear morphological changes in HepG2 cells after treatment with 2 × IC50 of crude extracts for 24 hours followed by DAPI staining. (A) Control cells treated with media; (B) melphalan-treated group (80 μg/ml). (C-H) cells treated with extracts of: (C) G. daltonii (220 μg/ml); (D) C. orientalis (500 μg/ml); (E) C. speciosum (110 μg/ml); (F) A. villosum (240 μg/ml); (G) A. tatarinowii (500 μg/ml); and (H) Pinus kesiya (110 μg/ml). Arrows indicate apoptotic bodies of nuclear fragmentation observed at 40× magnification under an inverted fluorescence microscope.