Forensically informative nucleotide sequencing (FINS) for the authentication of Chinese medicinal materials

Chinese Medicine

Table 1 Universal primers for PCR amplification of commonly used DNA regions

Region	Primer (5' > 3')		Reference
5S rDNA spacer	S-1	GGATTCGTGCTTGGGCGAGAGTAGTA	[34]
	AS-1	TGCGATCATACCAGCACTAAGGATCC
12S rDNA	Fwd	CAAACTGGGATTAGATACCCCACTAT	[35]
	Rev	GAGGGTGACGGGCGGTGTGT
16S rDNA	Fwd	CGCCTGTTTATCAAAAACAT	[36]
	Rev	CTCCGGTTTGAACTCAGATC
18S rDNA	18SF	CAACCTGGTTGATCCTGCCAGT	[37]
	18SR	CTGATCCTTCTGCACCTTCACCTAC
COI	LCO1490	GGTCAACAAATCATAAAGATATTGG	[15]
	HCO2198	TAAACTTCAGGGTGACCAAAAAATCA
Cyt b	mcb398	TACCATGAGGACAAATATCATTCTG	[38]
	Rev	CCTCCTAGTTTGTTAGGGATTGATCG
ITS	ITS4	TCCTCCGCTTATTGATATGC	[18]
	ITS5a	CCTTATCATTTAGAGGAAGGAG
ITS-1	18d	CACACCGCCCGTCGCTCCTACCGA	[39]
	5.8c	TTGCGTTCAAAGACTCGATG
ITS-2	5.8d	AACCATCGAGTCTTTGAACGCA	[39]
	28cc	ACTCGCCGTTACTAGGGGAA
MatK	3F_KIM f	CGTACAGTACTTTTGTGTTTACGAG	[19]
	1R_KIM r	ACCCAGTCCATCTGGAAATCTTGGTTC
Mitochondrial control region	L15998	TACCCCAAACTCCCAAAGCTA	[40]
	CSBDH	TGAATTAGGAACCAGATGCCAG
TrnH-psbA	psbA3'f	GTTATGCATGAACGTAATGCTC	[18]
	trnHf	CGCGCATGGTGGATTCACAATCC
TrnL-trnF	Tab C	CGAAATCGGTAGACGCTACG	[41]
	Tab F	ATTTGAACTGGTGACACGAG
RbcL	rbcLa_F	ATGTCACCACAAACAGAGACTAAAGC	[19]
	rbcLa_R	GTAAAATCAAGTCCACCRCG

ISSN: 1749-8546