IL-4 receptor expression in peritoneal macrophages after EA treatment. Mice were treated with EA (15/30 Hz) at the Zusanli acupoint (ST36) for 20 min/d for 5 d. After the final EA session, the mice were euthanized and their peritoneal cells were harvested and stained for CD11b and IL-4 receptor. (A and C) Dot blot analyses of total peritoneal cells. (B and D) IL-4 receptor expression in CD11b+ cells. The percentages of IL-4 receptor + cells in CD11b+ cells (E) and mean fluorescence intensity of IL-4 receptor expression in CD11b+ cells (F) are also shown for three independent experiments containing three mice in each group. The sham-treated mice received only needle insertion into a non-acupoint (gluteal muscle) without electrical stimulation.