Panel |
H. androsaemum
|
H. kouytchense
|
H. maculatum
|
H. athoum
|
H. calycinum
|
H. ascyron
|
H. perforatum
|
---|
Non-and | x | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
Non-ath | ✓ | ✓ | ✓ | x | ✓ | ✓ | ✓ |
Non-asc | ✓ | ✓ | ✓ | ✓ | ✓ | x | ✓ |
Non-perf | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | x |
Multiplex | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
- The initial PCR products were diluted by 1 × 10-5 (except H. athoum, 1 × 10-4) and equal volumes of each sample were added to the panel mixtures, as indicated by a tick. The panels are described by the name of the species that is not present, indicated by a cross. These panels were then used to test the species-specific primers for each target for cross-amplification, allowing six DNA samples to be tested simultaneously. The panel used to test the multiplex reaction contained all the available samples, and is named Multiplex.