Table 3 Candidate primer pairs for species-specific identification of the target DNAs
From: PlantID – DNA-based identification of multiple medicinal plants in complex mixtures
Forward primer name | Sequence 5′ to 3′ | Tm | Reverse primer name | Sequence 5′ to 3′ | Tm | Amplicon length (bp) | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Hand | F | 1 | 2 | ACATCGTCGCCCCAAACC | 67.0 | Hand | R | 1 | 2 | CCATTATCCGCCCCATCCTC | 66.5 | 65 |
Hand | F | 1 | 3 | AAATGTGATACTTGGTGTGAATTGC | 64.7 | Hand | R | 1 | 3 | CGAGGTGTTGGGTTTGGG | 64.8 | 135 |
Hand | F | 1 | 4 | CACATCGTCGCCCCAAAC | 65.6 | Hand | R | 1 | 4 | ACCATTATCCGCCCCATCC | 65.9 | 67 |
Hand | F | 1 | 4 | CACATCGTCGCCCCAAAC | 65.6 | Hand | R | 1 | 5 | TTATCCGCCCCATCCTCTTC | 65.4 | 63 |
Hand | F | 1 | 5 | CGGCTGTCCTCCTGTTCATAAC | 67.1 | Hand | R | 1 | 6 | TCACACCAAGTATCACATTTCGCTAC | 67.3 | 98 |
Hand | F | 2 | 1 | CGAAATGTGATACTTGGTGTGAATTG | 65.1 | Hand | R | 1 | 3 | CGAGGTGTTGGGTTTGGG | 64.8 | 137 |
Hasc | F | 1 | 2 | TTCCTTCGGTTCATAACTAAAAC | 60.9 | Hasc | R | 1 | 2 | ACCCAATGAACTCGAAAGAG | 61.7 | 225 |
Hasc | F | 1 | 4 | GTGGCTTTCCTTCGGTTC | 62.5 | Hasc | R | 1 | 2 | ACCCAATGAACTCGAAAGAG | 61.7 | 231 |
Hasc | F | 1 | 3 | TTTCCTTCGGTTCATAACTAAAAC | 61.5 | Hasc | R | 1 | 1 | GAACTCGAAAGAGGCATTG | 60.4 | 219 |
Hasc | F | 1 | 5 | TCCTTCGGTTCATAACTAAAAC | 60.2 | Hasc | R | 1 | 1 | GAACTCGAAAGAGGCATTG | 60.4 | 217 |
Hath | F | 1 | 1 | CCCCGAAATTCCGATATCTC | 61.8 | Hath | R | 1 | 1 | CTTACAACCACCGCTAGTC | 61.7 | 137 |
Hath | F | 1 | 1 | CCCCGAAATTCCGATATCTC | 61.8 | Hath | R | 1 | 3 | CAACCACCGCTAGTCGTG | 64.6 | 133 |
Hath | F | 1 | 1 | CCCCGAAATTCCGATATCTC | 61.8 | Hath | R | 1 | 4 | CCGCTAGTCGTGGCTTTG | 64.9 | 127 |
Hath | F | 1 | 3 | GTGTCACACATCGTTGCC | 63.2 | Hath | R | 1 | 3 | CAACCACCGCTAGTCGTG | 64.6 | 151 |
Hper | F | 1 | 1 | TGTAACGCTCCCGGCTGTG | 69.0 | Hper | R | 1 | 1 | CCGATTGTCTCTTGCGAGATATC | 65.0 | 273 |
Hper | F | 4 | 1 | GGGGCTTCCTTCTGTTCATAAC | 65.1 | Hper | R | 4 | 1 | TCTTGCGAGATATCGGGATTTTG | 64.9 | 222 |
Hper | F | 1 | 2 | ATAAGAAGTGTAACGCTCCCGGCTGTG | 72.5 | Hper | R | 1 | 1 | CCGATTGTCTCTTGCGAGATATC | 65.0 | 281 |
Hper | F | 1 | 3 | GAAGTGTAACGCTCCCGGCTGTG | 71.9 | Hper | R | 1 | 1 | CCGATTGTCTCTTGCGAGATATC | 65.0 | 277 |
Hper | F | 1 | 4 | AGTGTAACGCTCCCGGCTGTG | 71.2 | Hper | R | 1 | 1 | CCGATTGTCTCTTGCGAGATATC | 65.0 | 275 |