Figure 1

Inhibition of TNF-α and IL-4 secretion from U937 and EL4 cells by α-MG. (A) U937 cells were treated with 0.1 ng/mL LPS in the presence or absence of different concentrations of α-MG, 7.6, 12.5, 30.5 nM, respectively for 4 h. TNF-α secreted into the conditioned media was quantified by ELISA. The TNF-α content (gray bars) and cell viabilities (open bars) are shown. (B) EL4 cells were treated with 0.1 ng/mL LPS in the presence or absence of different concentrations of α-MG, 3.0, 6.1, 12.2 nM, respectively for 18 h. IL-4 secreted into the conditioned media was quantified by ELISA. The IL-4 content (gray bars) and cell viabilities (open bars) are shown. All experiments were performed in triplicate and repeated independently three times. *P < 0.05, significant difference from LPS treatment.