Figure 6

α-MG regulates STAT 1, c-Jun and c-Fos. U937 cells were treated with α-MG in the presence of 0.1 ng/mL LPS for 4 h and then lysed. The cell lysates were subjected to Western blotting analyses with STAT 1, c-Jun, c-Fos. Western blots with anti-phospho- STAT 1, anti-phospho- c-Jun, and anti-phospho- c-Fos. β-tubulin was evaluated as a loading control, and the protein expression levels were normalized by the corresponding β-tubulin expression levels. Data are expressed as fold phosphorylation normalized to LPS (12 nM α-MG, closed bars; 6 nM α-MG, open bars). All experiments were performed in triplicate and repeated independently three times. *P < 0.05, significant difference from LPS treatment.