Protective effect of Phellinus linteus polysaccharide extracts against thioacetamide-induced liver fibrosis in rats: a proteomics analysis

Table 1 Primers used for the PCR analyses

Gene	Primer sequence (5’-3’)	Fragment size (bp)	Annealing temperature (°C)
Preprohaptoglobin	F¹: TGCCTATCTGCCTGCCTTC	316	58
Preprohaptoglobin	R²: GTGTCCTCCTCCGTGTCAT	316	58
Hemopexin	F: AAGCCAGACTCAGATGTAA	479	55
Hemopexin	R: AAGCAGTAGTAGCGTTCA	479	55
Gsta4	F: GGACCTGATGATGATGATTATC	446	54
Gsta4	R: TATCTTGCCTCTGGAATGC	446	54
Bckdha	F: AGCGTCACTTCGTCACCATT	547	60
Bckdha	R: GCCTTCTCCTGTTCCTCATCC	547	60
Bhmt	F: CAGACACCTTCCTACCTCAG	281	52
Bhmt	R: CAGTTCACACCGACAATGG	281	52
Dhfr	F: CTTGACGGCACTCTAAGC	304	52
Dhfr	R: CTCCTTGTGGTGGTTCCT	304	52
Qdpr	F: GATGTGGTGGAGAATGAAGAGG	241	56
Qdpr	R: AGTGGCTAGAGATGGTGGATG	241	56
Gapdh	F: CATGACCACAGTCCATGCCATC	451	60
Gapdh	R: CACCCTGTTGCTGTAGCCATATTC	451	60
Uk114	F: GCATGTCGTCAATAATCAGA	443	54
Uk114	R: CTCCAGAGTCAGCATCAG	443	54
Tft	F: GGTTCATCAGGTGCTCTATCG	311	58
Tft	R: CCAGGTCGTCTCCATCGTATA	311	58
Ubiquitin C	F: TGGAGGTCGAGCCCAGTGTTA	105	58
Ubiquitin C	R: CCCAAGAACAAGCACAAGAAGGGCT	105	58

ISSN: 1749-8546