Cell viability and neuroprotection against reserpine-induced injury. (A–C) Using the MTT cell proliferation assay, fluoxetine and different concentrations of XYS and XYS/RTSES were evaluated for cell proliferation (A). Various concentrations of reserpine were evaluated for damage to glial cells (B). Pretreatment with reserpine (100 M) at 24 h followed by treatment with fluoxetine, XYS, and XYS/RTSES (C) on the glioma C6 cell line after incubation for 24 and 72 h is shown. Each value is expressed as the mean ± SD of three culture wells. *P < 0.05, **P < 0.01, ***P < 0.001, significant difference compared with control C6 cells. #P < 0.05, ##P < 0.01, ###P < 0.001, significantly difference compared with XYS alone.