Figure 4

Zedoary oil induces cycle arrest in AGS cells. Zedoary oil was added at the indicated concentrations and the cells were incubated for 6, 12, 24 and 48 h. The cell cycle proportions were determined by flow cytometry after staining with PI. (A–D) Statistical analyses of the cell cycle phase distributions after 6, 12, 24 and 48 h, respectively. (E) Typical pictures of the respective cell cycle phase distributions from flow cytometry: a1–a4, control, 30, 60 and 90 μg/mL for 6 h; b1–b4, control, 30, 60 and 90 μg/mL for 12 h; c1–c4, control, 30, 60 and 90 μg/mL for 24 h; d1–d4, control, 30, 60 and 90 μg/mL for 48 h. The data represent means ± SD (*P < 0.05, **P < 0.01 vs. control cells). The control cells were AGS cells cultured in Ham’s/F-12 medium containing 10% FBS and 0.1% DMSO.