Figure 5

Effects of essential oil of zedoary on cell apoptosis of AGS cells at 24 h. (IA) Control; (IB) 60 μg/mL; (IC) 90 μg/mL; (ID) 120 μg/mL; (IE) effects of essential oil of zedoary on cell apoptosis evaluated by annexin V/PI double-staining at 24 h. (II) Effects of zedoary oil on AGS cell apoptosis and necrosis evaluated by Hoechst 33342/PI double-staining for 24 h (200×). (III) Ratios of Hoechst 33342-positive cells whose blue color staining was brilliant and aggregative. The control cells were AGS cells cultured in Ham’s/F-12 medium containing 10% FBS and 0.1% DMSO. *P < 0.05, **P < 0.01 vs. control cells.