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Table 5 Sequence divergence of the seven barcoding regions of Gentiana and P. hexandrum

From: Evaluation of seven DNA barcodes for differentiating closely related medicinal Gentiana species and their adulterants

 

rbcL

matK

trnH-psbA

trnL-F

rpl36-rps8

ITS

5S rRNA

(1) Comparison between (A) Medicinal Gentiana & (B) P. hexandrum

i) Minimum sequence divergence between Medicinal Gentiana & P. hexandrum

0.0995

0.3399

0.3908

0.3888

0.2211

0.4781

0.6154

ii) Maximum intraspecific divergence among Medicinal Gentiana & P. hexandrum

0.0018

0.0042

0.0101

0.0026

0.0033

0.0058

0.0939

iii) Can this barcode distinguish these two groups? #

Yes

Yes

Yes

Yes

Yes

Yes

Yes

iv) Differentiation power ranking*

7

5

3

4

6

2

1

(2) Comparison between (A) Medicinal Gentiana & (B) G. rhodantha

i) Minimum sequence divergence between Medicinal Gentiana & G. rhodantha

0.0128

0.0597

0.1349

0.1562

0.0958

0.0862

0.3098

ii) Maximum intraspecific divergence among Medicinal Gentiana & G. rhodantha

0.0018

0.0042

0.0101

0.0026

0.0033

0.0058

0.0914

iii) Can this barcode distinguish these two groups? #

Yes

Yes

Yes

Yes

Yes

Yes

Yes

iv) Differentiation power ranking*

7

6

3

2

4

5

1

(3) Comparison between (A) Guanlongdan & (B) Jianlongdan

i) Minimum sequence divergence between Guanlongdan & Jianlongdan

0.0109

0.0521

0.0780

0.0332

0.0392

0.0462

0.4897

ii) Maximum intraspecific divergence among Guanlongdan & Jianlongdan

0.0018

0.0042

0.0101

0.0026

0.0000

0.0043

0.0914

iii) Can this barcode distinguish these two groups?#

Yes

Yes

Yes

Yes

Yes

Yes

Yes

iv) Differentiation power ranking*

7

3

2

6

5

4

1

  1. #Barcode is able to distinguish the two groups if (i) > (ii).
  2. *The differentiation power ranking was determined by (i). The most varied locus was ranked as 1 and the least varied locus was ranked as 7.