Puerarin inhibites adipogenesis in 3T3-L1 cells without cell toxicity. (A) Puerarin had no toxic effect on 3T3-L1 preadipocyte cells on the tested concentrations. (B) Puerarin inhibited postconfluent mitotic clonal expansion in 3T3-L1 preadipocytes after 48 hour treatment. (C) Puerarin inhibited adipogenesis-induced accumulation of lipids in 3T3-L1 preadipocytes at day 8 after adipogenic induction. Representative morphological changes of 3T3-L1 adipocyte differentiation were by Oil Red O staining. (D) Puerarin suppressed triglyceride accumulation during adipogenesis.