Table 1 HPLC-ESI-MS and corresponding CID data of ginsenoside standards and the metabolites of ginsenoside Rb1 by human gut bacteria
From: Biotransformation of ginsenoside Rb1 via the gypenoside pathway by human gut bacteria
Retention time (min) | MS ( m/z) | CID ( m/z) | Identity | ||
|---|---|---|---|---|---|
[M-H]- | [M + Cl]- | [M + AcO]- | |||
20.9 | 799 | 835 | 859 | 637[M-H-Glc]-, 619[M-H-Glc-H2O]-, 475[M-H-2Glc]- | Ginsenoside Rg1 (IS) |
33.2 | 1107 | 1143 | - | 945[M-H-Glc]-, 783[M-H-2Glc]-, 621[M-H-3Glc]- | Ginsenoside Rb1 |
35.1 | 945 | 981 | - | 783[M-H-Glc]-, 621[M-H-2Glc]-, | Ginsenoside Rd (M1) |
35.6 | 945 | 981 | - | 783[M-H-Glc]-, 621[M-H-2Glc]-, | Gypenoside XVII (M2) |
38.2 | 783 | 819 | 843 | 621[M-H-Glc]-, 459[M-H-2Glc]- | Ginsenoside F2 (M3) |
39.7 | 783 | 819 | 843 | 621[M-H-Glc]-, 459[M-H-2Glc]- | Gypenoside LXXV (M4) |
40.3 | 783 | 819 | - | 621[M-H-Glc]-, 459[M-H-2Glc]- | Ginsenoside Rg3 |
45.4 | 621 | 657 | 681 | 459[M-H-Glc]- | Ginsenoside Rh2 |
44.4 | 621 | 657 | 681 | 459[M-H-Glc]- | Compound K (M5) |