Figure 1

THP-1 cells cultivated for 24 h with PMA (a), no addition (b) and 1 mg/mL A. bisporus polysaccharide extract (c); comparison of phagocytotic activity after PMA treatment (d) and A. bisporus polysaccharide extract treatment (e). Cells were cultivated in a 24 wells plate, washed with PBS, fixated in 4% formaldehyde and stained by crystal violet. Figure 1a shows that PMA treated cells form aggregates after 24 h incubation and adhere to the plate. Figure 1b shows that non treated control cells do not adhere and are washed away. Figure 1c shows the effect of A. bisporus polysaccharide extract, stretching of the originally rounded cells, and adherence to the plate. Figure 1d shows the uptake of fluorescent latex particles in PMA treated THP-1 cells and Figure 1e shows uptake of particles in A. bisporus polysaccharide extract treated cells.