Figure 5
Protective effect of human serum against P. linteus polysaccharide extract induced cell death in THP-1 cells (b) compared with the effects of varying P. linteus polysaccharide extract concentrations (a). THP-1 cells were cultivated for 24 h in complete RPMI medium with 100 μg/mL P. linteus polysaccharide extract to which increasing amounts of human serum was added. Cells were stained with trypan blue and non-colored cells were considered "live". Blue cells were considered "dead". A minimum of 1000 cells was counted per point. Figure 5a shows concentration-dependent cytotoxicity of P. linteus polysaccharide extract. In the right section of the figure the effect is shown of simultaneous addition of 100 μL of human serum. Figure 5b shows the concentration-dependent inhibition of P. linteus polysaccharide extract cytotoxicity by human serum respectively by decomplemented serum. *P < 0.01, **P < 0.05, significantly different from the values of the previous (lower) concentration in the range; 0P > 0.05 not different between the concentrations indicated.