Figure 7

FCM histograms of THP1 cells showing cellular DNA distributions after various treatments. Shown are the histograms at 48 h of control (a), P. linteus polysaccharide extract (b), P. linteus polysaccharide extract plus HS (c), PMA (d), A. bisporus polysaccharide extract (e) and A. brasiliensis polysaccharide extract (f). Fluorescence intensity of PI is presented on the logarithmic horizontal axis, cell count on the vertical axis. Histogram from cells is gated in FS and SS dotplot to exclude cell aggregates and cellular debris. Treatment with P. linteus polysaccharide extract results in cell cycle arrest in G1, with a skewed slope to sub G1 levels due to fragmenting nuclei and dead cells, indicated by the solid arrow in (b). The cell cycle arrest is overcome by cotreatment with HS, resulting in a resumption of DNA synthesis, indicated by the open arrow in (c). In the lower row, extracts from A. bisporus and A. brasiliensis result in comparable DNA profiles as PMA (d) with strong increases of the numbers of cells in the S-phase (open arrows).