Figure 9

PCA of DHE and JC-1 stained THP-1 cells. Data from DHE and JC-1 stained cells were gated on forward and sideward scatter and processed by StatistiXl plug in for Excel. Selected parameters are: JC1 fluorescence intensity histogram: Median (Median_JC1), CV (CV_JC1); DHE fluorescence intensity histogram: median (Median_DHE), CV (CV_DHE); forward scatter on DHE signal histogram: median (FS_median_DHE), CV (CV_FS_DHE), sideward scatter: Median (Median_SS_DHE), CV (CV_SS_DHE); pulse time on DHE signal: mean (Mean_PT_DHE), CV (CV_PT_DHE) and% of total (%_PT_DHE). All eigenvectors computed from these parameters are indicated on the plot by the small squares, marked with the related vector code. The big arrows indicate the vectors for the observed cellular properties, extracted from the PCA. The marked populations comprise the different treatments, with each dot an individual treatment. Treatments are mentioned in the text, and are connected by the solid lines, the arrowheads indicate the increasing concentration of the extracts applied. A minimum of 3000 cells in the FS, SS gate was counted.