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Table 3 Calibration curves, LOD and LOQ data of investigated compounds by HPLC-DAD

From: Chemical fingerprinting and quantitative constituent analysis of Siwu decoction categorized formulae by UPLC-QTOF/MS/MS and HPLC-DAD

Analytes Calibration curvesa r 2 Linear range (μg/mL) LOD (μg/mL) LOQ (μg/mL)
Gallic acid Y = 2.4 × 106X-8022.9 0.9999 148.0-1480.0 7.5 14.1
Protocatechuic acid Y = 8.0 × 106X-36350 0.9994 38.4-384.0 8.6 19.2
Vanillic acid Y = 2.6 × 106X-18490 0.9997 32.0-320.0 5.0 10.0
Caffeic acid Y = 3.5 × 106X-25506 1.0000 35.6-356.0 6.5 13.6
Paeoniflorin Y = 1.1 × 106X + 160000 0.9990 476.0-4760.0 24.8 59.6
Ferulic acid Y = 1.1 × 107X-1.2 × 105 0.9997 136.0-1360.0 6.2 12.4
Senkyunolide I Y = 6.5 × 106X-9533.5 0.9999 46.0-460.0 6.3 12.6
  1. a y is the logarithmic value of peak area and x is the logarithmic value of the reference compound’s concentration (μg/ml).