Skip to main content

Table 3 Calibration curves, LOD and LOQ data of investigated compounds by HPLC-DAD

From: Chemical fingerprinting and quantitative constituent analysis of Siwu decoction categorized formulae by UPLC-QTOF/MS/MS and HPLC-DAD

Analytes

Calibration curvesa

r 2

Linear range (μg/mL)

LOD (μg/mL)

LOQ (μg/mL)

Gallic acid

Y = 2.4 × 106X-8022.9

0.9999

148.0-1480.0

7.5

14.1

Protocatechuic acid

Y = 8.0 × 106X-36350

0.9994

38.4-384.0

8.6

19.2

Vanillic acid

Y = 2.6 × 106X-18490

0.9997

32.0-320.0

5.0

10.0

Caffeic acid

Y = 3.5 × 106X-25506

1.0000

35.6-356.0

6.5

13.6

Paeoniflorin

Y = 1.1 × 106X + 160000

0.9990

476.0-4760.0

24.8

59.6

Ferulic acid

Y = 1.1 × 107X-1.2 × 105

0.9997

136.0-1360.0

6.2

12.4

Senkyunolide I

Y = 6.5 × 106X-9533.5

0.9999

46.0-460.0

6.3

12.6

  1. a y is the logarithmic value of peak area and x is the logarithmic value of the reference compound’s concentration (μg/ml).