Effect of amygdalin on follistatin expression and cell proliferation in skeletal muscle C2C12 cells. (A) Induction of follistatin mRNA by amygdalin. Mouse skeletal muscle C2C12 cells were treated with amygdalin at the indicated concentrations for 24 h. The levels of follistatin mRNA were determined by RT-PCR. (B) Stimulation of skeletal muscle cell growth by amygdalin. C2C12 cells were treated with amygdalin at the indicated concentrations for 24 or 48 h. The viable cells were measured by a standard MTT assay. The values represent means ± SD from three independent experiments. *P < 0.05, **P < 0.01, amygdalin treatment vs. control.