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Table 1 Mean inhibition zones of the B. marginatum extracts against different pathogens determined by the agar diffusion method

From: Anti-infective and cytotoxic properties of Bupleurum marginatum

Microorganisms Diameter of inhibition zone (mm)
CH3OH extract 640 μg/well CH2Cl2extract 640 μg/well Ampicillin 10 μg/well Vancomycin 10 μg/well Nystatin 10 μg/well
Gram positive      
 MRSA 818014 NI NI 5.3 ± 0.3 10.3 ± 0.7 NT
 MRSA 818081 NI NI 7.2 ± 0.0 10.6 ± 1.3 NT
 MRSA NCTC 10442 NI NI NI 10.0 ± 0.2 NT
Staphylococcus aureus ATCC 29213 4.3 ± 0.7 (P*1,2 = 0.001, 0.008) 5.1 ± 1.4 (P1,2 = 0.001, 0.008) 24.0 ± 1.5 10.7 ± 1.6 NT
Staphylococcus epidermidis ATCC 14990 4.3 ± 2.5 (P*1,2 = 0.016, 0.027) 5.6 ± 1.3 (P1,2 = 0.021, 0.019) 12.2 ± 1.7 10.0 ± 0.5 NT
Streptococcus pyogenes ATCC 12344 5.7 ± 1.6 (P*1,2 = 0.006, 0.021) 7.2 ± 2.1 (P1,2 = 0.011, 0.017) 25.0 ± 1.2 15.3 ± 1.6 NT
Streptococcus agalactiae ATCC 27956 5.2 ± 1.1 (P*1,2 = 0.024, 0.021) 5.2 ± 1.5 (P1,2 = 0.023, 0.021) 17.1 ± 1.5 12.0 ± 1.7 NT
Bacillus subtilis ATCC 6051 NI 4.2 ± 2.1 (P1,2 = 0.012, 0.038) 12.7 ± 0.5 8.7 ± 1.2 NT
Gram negative      
Pseudomonas aeruginosa ATCC 27853 4.1 ± 0.0 5.3 ± 1.3 NI NI NT
Escherichia coli ATCC 25922 4.3 ± 0.2 NI 5.4 ± 1.2 NI NT
Fungi      
Candida albicans ATCC 90028 NI NI NT NT 10.0 ± 1.2
Candida glabrata ATCC MYA 2950 NI NI NT NT 12.1 ± 1.2
  1. Data are presented as means ± SD (n = 3).
  2. NI: no inhibition zone; NT: not tested.
  3. P1,2: Significant differences relative to ampicillin and vancomycin, respectively.