Anti-infective and cytotoxic properties of Bupleurum marginatum

Chinese Medicine

Table 1 Mean inhibition zones of the B. marginatum extracts against different pathogens determined by the agar diffusion method

Microorganisms	Diameter of inhibition zone (mm)
Microorganisms	CH₃OH extract 640 μg/well	CH₂Cl₂extract 640 μg/well	Ampicillin 10 μg/well	Vancomycin 10 μg/well	Nystatin 10 μg/well
Gram positive
MRSA 818014	NI	NI	5.3 ± 0.3	10.3 ± 0.7	NT
MRSA 818081	NI	NI	7.2 ± 0.0	10.6 ± 1.3	NT
MRSA NCTC 10442	NI	NI	NI	10.0 ± 0.2	NT
Staphylococcus aureus ATCC 29213	4.3 ± 0.7 (P*^1,2 = 0.001, 0.008)	5.1 ± 1.4 (P^1,2 = 0.001, 0.008)	24.0 ± 1.5	10.7 ± 1.6	NT
Staphylococcus epidermidis ATCC 14990	4.3 ± 2.5 (P*^1,2 = 0.016, 0.027)	5.6 ± 1.3 (P^1,2 = 0.021, 0.019)	12.2 ± 1.7	10.0 ± 0.5	NT
Streptococcus pyogenes ATCC 12344	5.7 ± 1.6 (P*^1,2 = 0.006, 0.021)	7.2 ± 2.1 (P^1,2 = 0.011, 0.017)	25.0 ± 1.2	15.3 ± 1.6	NT
Streptococcus agalactiae ATCC 27956	5.2 ± 1.1 (P*^1,2 = 0.024, 0.021)	5.2 ± 1.5 (P^1,2 = 0.023, 0.021)	17.1 ± 1.5	12.0 ± 1.7	NT
Bacillus subtilis ATCC 6051	NI	4.2 ± 2.1 (P^1,2 = 0.012, 0.038)	12.7 ± 0.5	8.7 ± 1.2	NT
Gram negative
Pseudomonas aeruginosa ATCC 27853	4.1 ± 0.0	5.3 ± 1.3	NI	NI	NT
Escherichia coli ATCC 25922	4.3 ± 0.2	NI	5.4 ± 1.2	NI	NT
Fungi
Candida albicans ATCC 90028	NI	NI	NT	NT	10.0 ± 1.2
Candida glabrata ATCC MYA 2950	NI	NI	NT	NT	12.1 ± 1.2

Data are presented as means ± SD (n = 3).
NI: no inhibition zone; NT: not tested.
P^1,2: Significant differences relative to ampicillin and vancomycin, respectively.

ISSN: 1749-8546