Inhibitory effects of Eucommia lignans on Ang II-induced ECM biosynthesis in RMCs. (A) depression of Eucommia lignans on Ang II-increased mRNA expression levels; (B) inhibition of Eucommia lignans on Ang II-stimulated protein expression levels. RT-qPCR and western blot was used to detect the mRNA and protein expressions separately. GAPDH was used as an internal loading control gene. Both mRNA and protein relative expression levels were expressed as folds of control. All values were expressed as mean ± SD (n = 3). Los: losartan; Lig: lignans. ##P < 0.01 vs. the control group, **P < 0.01 vs. Ang II group.