Inhibitory effects of Eucommia lignans on Ang II-induced AR expression in RMCs. (A) Inhibition of Eucommia lignans on Ang II-increased AR mRNA levels; (B and C) depression of Eucommia lignans on Ang II-stimulated AR protein expression. Cells were incubated as described in Methods. RT-qPCR and Western blotting was used to detect the mRNA and protein expressions. GAPDH was selected as an internal standard gene. AR relative expression levels were indicated as folds of control. All results were shown in mean ± SD (n = 3). Los: losartan; Lig: lignans. ##P < 0.01 vs. the control group, **P < 0.01 vs. Ang II group.