PCR amplification and DNA concentration in decoction with sliced or pulverized sample at different times of boiling. (A)
P. ginseng sample was sliced (lanes 1–3, 7–9) or pulverized (lanes 4–6, 10–12) and boiled for 30 min (lanes 1, 4, 7, 10), 60 min (lanes 2, 5, 8, 11) and 120 min (lanes 3, 6, 9, 12). Crude DNA (lanes 1–6) and extracted DNA with concentration adjusted to that in the original decoction (lanes 7–12) were amplified by primer pair 9. Lane 13 is the positive control with DNA from P. ginseng and lane 14 is the negative control without DNA. Lane M represents the DNA size ladder. (B) The concentration of extracted P. ginseng DNA was adjusted to make it similar to that in the original decoction and measured by Qubit 2.0 Fluorometer. Water was used as blank and the data represent mean ± standard deviation (n = 3). Difference between boiling time was analyzed by one-way analysis of variance. Difference between sliced and pulverized samples at the same boiling time was analyzed by two sample t test (*p < 0.05).