Table 2 Primers used for PCR amplification and sequencing
From: Identification of constituent herbs in ginseng decoctions by DNA markers
Herbal species | Primer pair | Primer name | Direction | Primer sequence (5′ to 3′) | Amplicon size (bp) | Annealing temperature (°C) | Gene or spacer region |
|---|---|---|---|---|---|---|---|
A. carmichaeli | 1 | Aca_F1 | Forward | GGTGCATGTCTGGCTTAG | 148 | 66 | trnH-psbA |
Aca_R1 | Reverse | TCACCAAACCAACACCAATC | |||||
A. macrocephala | 2 | Ama_F1 | Forward | CGACCCGCGAACATGTAAC | 108 | 56 | ITS2 |
Ama_R1 | Reverse | CGAGGCCCCGATAGGTG | |||||
G. uralensis | 3 | Gur_F1 | Forward | ACAGACCGTTGCCCGAC | 106 | 57 | ITS2 |
Gur_R1 | Reverse | CAGTTTTGAGCCAACCGTGAG | |||||
P. ginseng | 4 | Pgi_F1 | Forward | GGCAGTTGGCTAATGAAAGGTTGTA | 88 | 64 | 26S-18S |
Panax_R1 | Reverse | AAGCACCGCTATGCGCGAA | |||||
5 | Pgi_F2 | Forward | TGAAAGGTTGTAATAGTTT | 249 | 48 | 26S-18S | |
Pgi_R1 | Reverse | AATGAAAAGGAATGAAAG | |||||
6 | Pgi_F3 | Forward | ACGGTTGCTTTTCCATCATTGTGT | 311 | 64 | 26S-18S | |
Panax_R1 | Reverse | AAGCACCGCTATGCGCGAA | |||||
7 | Pgi_SPF1 | Forward | TGTCGGGCAAGGCCAAAAATG | 121 | 64 | 26S-18S | |
Panax_R1 | Reverse | AAGCACCGCTATGCGCGAA | |||||
8 | Panax_F1 | Forward | GGTGCTTTGAGTGCTGCTGA | 121 and 191 | 64 | 26S-18S | |
Pgi_SPF1 | Forward | TGTCGGGCAAGGCCAAAAATG | |||||
Panax_R1 | Reverse | AAGCACCGCTATGCGCGAA | |||||
P. ginseng, P. quinquefolius | 9 | Panax_F1 | Forward | GGTGCTTTGAGTGCTGCTGA | 191 | 64 | 26S-18S |
Panax_R1 | Reverse | AAGCACCGCTATGCGCGAA | |||||
Z. officinale | 10 | Zof_F1 | Forward | GTAAAAGTCGGCAGTCGC | 106 | 65 | ITS2 |
Zof_R1 | Reverse | CACGCAGGGTCTCTTGAGG |