Figure 3

Effects of LicA on (A) the LXRα-stimulated expression of lipogenic genes and (B) TG accumulation in mouse primary hepatocytes. (A) T0901317-induced expression of SREBP-1c, SCD1, FAS, and GPAT was determined in the presence or absence of LicA by qRT-PCR. Data were presented as means (SD) from four independent experiments with triplicate determinations. (B) TG accumulation in response to cotreatment with T0901317 and LicA. Intracellular TG levels were measured enzymatically after treatment of primary hepatocytes with 1 μM T0901317 and 10 μg/mL LicA and normalized to total protein levels. Data were presented as means (SD) from four independent experiments with duplicate determinations. Statistical analysis was performed by one-way ANOVA. P* = P value for Bonferroni correction. DMSO, dimethyl sulfoxide; T1317, T0901317.