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Table 2 Regression data, detection/quantification limits and precision data for the five compounds determined by UPLC-PDA

From: A multivariate analysis on the comparison of raw notoginseng (Sanqi) and its granule products by thin-layer chromatography and ultra-performance liquid chromatography

Compounds Regression equation R2 LOD LOQ Precision, RSD (%) Precision, RSD (%)
(μg/mL) (μg/mL) Intra-day (n = 3) Inter-day (n = 3)
  TLC* UPLC* TLC UPLC TLC UPLC TLC UPLC TLC UPLC TLC UPLC
NR1 y = 5752.1x + 796.27b y = 497892x + 1692a 0.993 0.994 16.60 3.77 49.80 11.30 3.17 1.95 1.04 4.59
Rb1 y = 15159x-498.43a y = 451285x-8794.1a 0.991 0.997 12.60 4.26 37.90 41.70 7.06 1.67 0.92 0.55
Rd y = 8877.8x + 1092.3b y = 502877x + 4048.3a 0.995 0.994 144.00 3.31 431.00 9.95 1.67 0.74 6.92 1.98
Rg1 y = 8095.7x + 1360.3a y = 519908x-3073.3a 0.992 0.993 126.00 16.20 378.00 48.80 2.23 3.58 4.68 2.82
Rg2 y = 11839x + 958.33a y = 529865x + 1901.5a 0.991 0.998 33.700 5.97 101.00 17.90 0.77 2.18 2.80 0.26
  1. RSD (%) = 100 × S.D./mean; y, peak area; x, the concentration of each reference compound (mg/mL); R2, coefficient of determination; LOD, limit of detection (3.33 × (SD of Y-intercept/mean of slope)); LOQ, limit of quantification (10 × (SD of Y-intercept/mean of slope))
  2. an=3
  3. bn=4
  4. *All P values for the regression equations are = 0.000