A multivariate analysis on the comparison of raw notoginseng (Sanqi) and its granule products by thin-layer chromatography and ultra-performance liquid chromatography

Table 2 Regression data, detection/quantification limits and precision data for the five compounds determined by UPLC-PDA

Compounds	Regression equation		R²		LOD		LOQ		Precision, RSD (%)		Precision, RSD (%)
Compounds	Regression equation		R²		(μg/mL)		(μg/mL)		Intra-day (n = 3)		Inter-day (n = 3)
	TLC*	UPLC*	TLC	UPLC	TLC	UPLC	TLC	UPLC	TLC	UPLC	TLC	UPLC
NR1	y = 5752.1x + 796.27^b	y = 497892x + 1692^a	0.993	0.994	16.60	3.77	49.80	11.30	3.17	1.95	1.04	4.59
Rb1	y = 15159x-498.43^a	y = 451285x-8794.1^a	0.991	0.997	12.60	4.26	37.90	41.70	7.06	1.67	0.92	0.55
Rd	y = 8877.8x + 1092.3^b	y = 502877x + 4048.3^a	0.995	0.994	144.00	3.31	431.00	9.95	1.67	0.74	6.92	1.98
Rg1	y = 8095.7x + 1360.3^a	y = 519908x-3073.3^a	0.992	0.993	126.00	16.20	378.00	48.80	2.23	3.58	4.68	2.82
Rg2	y = 11839x + 958.33^a	y = 529865x + 1901.5^a	0.991	0.998	33.700	5.97	101.00	17.90	0.77	2.18	2.80	0.26

RSD (%) = 100 × S.D./mean; y, peak area; x, the concentration of each reference compound (mg/mL); R², coefficient of determination; LOD, limit of detection (3.33 × (SD of Y-intercept/mean of slope)); LOQ, limit of quantification (10 × (SD of Y-intercept/mean of slope))
^an=3
^bn=4
*All P values for the regression equations are = 0.000

ISSN: 1749-8546